• About
  • Masthead
  • License Content
  • Advertise
  • Submit Press Release
  • RSS/Email List
  • 2MM Podcast
  • Write for us
  • Contact Us
2 Minute Medicine
No Result
View All Result

No products in the cart.

SUBSCRIBE
  • Specialties
    • All Specialties, All Recent Reports
    • Cardiology
    • Chronic Disease
    • Dermatology
    • Emergency
    • Endocrinology
    • Gastroenterology
    • Imaging and Intervention
    • Infectious Disease
    • Nephrology
    • Neurology
    • Obstetrics
    • Oncology
    • Ophthalmology
    • Pediatrics
    • Pharma
    • Preclinical
    • Psychiatry
    • Public Health
    • Pulmonology
    • Rheumatology
    • Surgery
  • AI Roundup
  • Pharma
  • The Scan+
  • Classics™+
    • 2MM+ Online Access
    • Paperback and Ebook
  • Rewinds
  • Visual
  • Podcasts
  • Partners
    • License Content
    • Submit Press Release
    • Advertise with Us
  • Account
    • Subscribe
    • Sign-in
    • My account
2 Minute Medicine
  • Specialties
    • All Specialties, All Recent Reports
    • Cardiology
    • Chronic Disease
    • Dermatology
    • Emergency
    • Endocrinology
    • Gastroenterology
    • Imaging and Intervention
    • Infectious Disease
    • Nephrology
    • Neurology
    • Obstetrics
    • Oncology
    • Ophthalmology
    • Pediatrics
    • Pharma
    • Preclinical
    • Psychiatry
    • Public Health
    • Pulmonology
    • Rheumatology
    • Surgery
  • AI Roundup
  • Pharma
  • The Scan+
  • Classics™+
    • 2MM+ Online Access
    • Paperback and Ebook
  • Rewinds
  • Visual
  • Podcasts
  • Partners
    • License Content
    • Submit Press Release
    • Advertise with Us
  • Account
    • Subscribe
    • Sign-in
    • My account
SUBSCRIBE
2 Minute Medicine
Subscribe
Home All Specialties Pediatrics

PCR associated with limited diagnostic utility in viral URI

byEmilia HermannandLeah Carr, MD
February 24, 2014
in Pediatrics, Public Health
Reading Time: 3 mins read
0
Share on FacebookShare on Twitter

Image: CDC/ Hsi Liu, Ph.D., MBA, James Gathany

1. Through use of quantitative PCR (qPCR) to evaluate viral infection in a cohort of pediatric patients with and without respiratory symptoms, researchers found that symptomatic patients were more likely to be infected with human bocavirus, parainfluenza, and metapneumovirus than asymptomatic patients. qPCR detection of common viruses such as rhinovirus and adenovirus was no more likely in symptomatic or asymptomatic patients.

2. Coinfection with more than 1 viral strain was more common in patients with respiratory symptoms than in control patients, and patients with symptoms and viral coinfection were more likely to have increased respiratory rate, tachycardia, and decreased oxygen saturation.

Evidence Rating Level: 3 (Average)

Study Rundown: Quantitative real-time polymerase chain reaction (qRT PCR) is a laboratory test that amplifies and identifies viral DNA or RNA. The use of PCR for diagnosis of viral infection has been introduced as a sensitive diagnostic tool in the clinic setting. This matched case-control study evaluated PCR as a diagnostic tool for acute respiratory infections (ARI). qRT PCR was used to identify viral isolates in nasopharyngeal samples from patients with and without upper respiratory infection symptoms. Researchers found that parainfluenza virus, human bocavirus, and metapneumovirus were more frequently found in patients with respiratory symptoms, while detection of corona virus, rhinovirus, and adenovirus were common among both case and control populations. Coinfection was more common among patients with symptoms than those without (20.1% and 5.3%, respectively), and patients with respiratory symptoms and coinfection were found to have more severe disease than those with a single infection. This study was limited by lack of follow-up of control studies after sampling, so early diagnosis of later infection is possible. Furthermore, the qRT PCR method employed in this study was not designed to evaluate viral load, a factor related to disease severity. This study suggested that qRT PCR may have limited utility in clinical diagnosis of respiratory infections because of the high prevalence of common viral strains in both symptomatic and asymptomatic individuals and because of the increased likelihood of viral coinfection in symptomatic patients.

Click to read the study in Pediatrics

RELATED REPORTS

SARS-CoV-2 detected on various surroundings of asymptomatic COVID-19 positive infant

Novel coronavirus identified from patients with pneumonia in Wuhan, China

Viral respiratory infections associated with increased risk of acute myocardial infarction

Relevant Reading: Infections of the lower respiratory tract: old viruses, new viruses, and the role of diagnosis

In-Depth [matched case-control study]: This study included a cohort of 229 children (mean age = 10 months, 59.3% male) less than 5 years old who presented to a Swedish pediatric emergency department with ARI symptoms (coryza, rhinorrhea, sore throat, cough, ear ache, or dyspnea) and matched them to a cohort of control patients without ARI symptoms. Nasopharyngeal aspirates were tested via qRT PCR for common viral strains. One hundred and fifty one (72.3%) case patients and 74 (35.4%) control patients had detectible viral infection. Human bocavirus, metapneumovirus, and parainfluenza were detected more frequently in case patients. Odds ratios (ORs) for infection in the case group for these viruses (adjusted for variables associated with ARI in initial univariate analyses) were 4.4 (95% CI: 1.6-11.8), 12.0 (95% CI: 2.0-71.6), and 33.6 (95% CI 3.8-294.4), respectively.  Forty-two (20.1%) patients with respiratory infection symptoms tested positive for more than 1 viral strain, with an unadjusted OR for coinfection of 12.3 (95% CI: 5.0 to 30.4). Patients with symptoms and viral coinfection were more likely to have increased respiratory rate (P = 0.009), tachycardia (P = 0.05), decreased oxygen saturation (P = 0.04), discharge diagnosis of pneumonia (P = 0.006), and bronchitis (P = 0.02). Patients later identified as having croup, bronchitis or asthma, were also most likely to test positive for more than 1 virus (88%).

More from this author: AAP urges bottle feeding over breastfeeding in mothers with HIV, Pediatric influenza burden remains high despite new vaccination recommendations, Cow’s milk consumption linked to increased vitamin D and decreased iron stores in early childhood

©2012-2014 2minutemedicine.com. All rights reserved. No works may be reproduced without expressed written consent from 2minutemedicine.com. Disclaimer: We present factual information directly from peer reviewed medical journals. No post should be construed as medical advice and is not intended as such by the authors, editors, staff or by 2minutemedicine.com. PLEASE SEE A HEALTHCARE PROVIDER IN YOUR AREA IF YOU SEEK MEDICAL ADVICE OF ANY SORT. 

Tags: PCRURIviral URI
Previous Post

2 Minute Medicine Rewind February 17 – February 23, 2014

Next Post

Weight loss may decrease urinary incontinence in male diabetics [Look AHEAD Study]

RelatedReports

SARS-CoV-2 detected on various surroundings of asymptomatic COVID-19 positive infant
Infectious Disease

SARS-CoV-2 detected on various surroundings of asymptomatic COVID-19 positive infant

April 18, 2020
Novel coronavirus identified from patients with pneumonia in Wuhan, China
Emergency

Novel coronavirus identified from patients with pneumonia in Wuhan, China

March 6, 2020
Live attenuated vaccine less effective in recent influenza season
Cardiology

Viral respiratory infections associated with increased risk of acute myocardial infarction

January 30, 2018
Tonsillectomy may have short-term benefits in recurrent throat infection
ENT

Tonsillectomy may have short-term benefits in recurrent throat infection

January 17, 2017
Next Post
Weight loss may decrease urinary incontinence in male diabetics [Look AHEAD Study]

Weight loss may decrease urinary incontinence in male diabetics [Look AHEAD Study]

Acetaminophen during pregnancy associated with ADHD-like behavior in children

Acetaminophen during pregnancy associated with ADHD-like behavior in children

Mediterranean diet linked to reduced diabetes risk in high risk cardiovascular patients

Partner bereavement linked to increase acute cardiovascular events

2 Minute Medicine® is an award winning, physician-run, expert medical media company. Our content is curated, written and edited by practicing health professionals who have clinical and scientific expertise in their field of reporting. Our editorial management team is comprised of highly-trained MD physicians. Join numerous brands, companies, and hospitals who trust our licensed content.

Recent Reports

  • Sodium-glucose cotransporter 2 inhibitors may decrease risk of serious liver events in patients with cirrhosis
  • #VisualAbstract: Lactated Ringer’s Solution Does Not Improve Outcomes Relative to Normal Saline
  • 2MM: AI Roundup – FDA’s AI Push, Trial Speedups with Real-World Data, Smart Surgical Monitors, and Regulatory Overhaul Begins [July 2nd, 2025]
License Content
Terms of Use | Disclaimer
Cookie Policy
Privacy Statement (EU)
Disclaimer

© 2021 2 Minute Medicine, Inc. - Physician-written medical news.

  • Specialties
    • All Specialties, All Recent Reports
    • Cardiology
    • Chronic Disease
    • Dermatology
    • Emergency
    • Endocrinology
    • Gastroenterology
    • Imaging and Intervention
    • Infectious Disease
    • Nephrology
    • Neurology
    • Obstetrics
    • Oncology
    • Ophthalmology
    • Pediatrics
    • Pharma
    • Preclinical
    • Psychiatry
    • Public Health
    • Pulmonology
    • Rheumatology
    • Surgery
  • AI Roundup
  • Pharma
  • The Scan
  • Classics™
    • 2MM+ Online Access
    • Paperback and Ebook
  • Rewinds
  • Visual
  • Podcasts
  • Partners
    • License Content
    • Submit Press Release
    • Advertise with Us
  • Account
    • Subscribe
    • Sign-in
    • My account
No Result
View All Result

© 2021 2 Minute Medicine, Inc. - Physician-written medical news.